Integrins are heterodimeric transmembrane receptors, consisting of α and β subunits, that are non-covalently associated they physically link the ECM to the intracellular actin cytoskeleton but are also able to transduce signals bidirectionally across the plasma membrane. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.Īdhesion of cells to extracellular matrix (ECM) is primarily mediated by integrins and is crucial for cell growth and survival. Languino, the corresponding author, is an editorial board member for PLOS ONE. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: Dr. This project is also funded, in part, under a Commonwealth University Research Enhancement Program grant with the Pennsylvania Department of Health (H.R.). This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: Contract grant sponsor: NIH Contract grant number: R01 CA-89720 and CA-109874 (to LRL), P01 CA-140043 (to LRL) American Cancer Society (ACS)-IRG-08-060-04 (to AS) Contract grant sponsor: Pennsylvania Department of Health. Received: JAccepted: AugPublished: October 9, 2013Ĭopyright: © 2013 Sayeed et al. PLoS ONE 8(10):Įditor: Natasha Kyprianou, University of Kentucky College of Medicine, United States of America Our results reveal a crucial mechanistic role for the α 5β 1 integrin, downstream of IGF-IR, in regulating cancer growth.Ĭitation: Sayeed A, Fedele C, Trerotola M, Ganguly KK, Languino LR (2013) IGF-IR Promotes Prostate Cancer Growth by Stabilizing α 5β 1 Integrin Protein Levels. The α 5 subunit, one of the binding partners of β 1, is also downregulated along with β 1 upon IGF-IR knockdown while no change is observed in the expression of the α 2, α 3, α 4, α 6 and α 7 subunits. We demonstrate that IGF-IR stabilizes the β 1 subunit by protecting it from proteasomal degradation. This appears to be due to the lack of interaction between the β 1 cytoplasmic domain and IGF-IR. Exogenous expression of a CD4 - β 1 integrin cytoplasmic domain chimera does not interfere with such regulation and fails to stabilize β 1 integrin expression in the absence of IGF-IR. We demonstrate that β 1 integrin regulation by IGF-IR does not occur at the mRNA level. Here we report that IGF-IR is crucial for PrCa cell growth and that β 1 integrins contribute to the regulation of proliferation by IGF-IR. We have dissected the mechanism through which IGF-IR regulates β 1 integrin expression in PrCa. Furthermore, we have recently reported that IGF-IR regulates the expression of β 1 integrins in PrCa cells. We have previously demonstrated that β 1 integrins regulate anchorage-independent growth of prostate cancer (PrCa) cells by regulating IGF-IR expression and androgen receptor-mediated transcriptional functions. The type 1 insulin-like growth factor receptor (IGF-IR) mediates tumor cell growth, adhesion and inhibition of apoptosis in several types of cancer. They also mediate signal transduction to regulate cell proliferation and survival. Integrins are transmembrane receptors that bind extracellular matrix proteins and enable cell adhesion and cytoskeletal organization. Dynamic crosstalk between growth factor receptors, cell adhesion molecules and extracellular matrix is essential for cancer cell migration and invasion.
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![]() ![]() Sonic Studio's Jon Reichbach reports, via email, "With MQA decode integration, Amarra 4 Luxe adds support for MQA playback of local MQA audio files and MQA Master files from Tidal HiFi streaming service. The player will have an entirely new look, and include integrated artwork. Amarra 4 Luxe, which is due in May 2017 from Sonic Studio, will support MQA decoding on OSX (10.8+) and Windows (8/10)-based computers for $99 pending final license agreement with MQA. Cost for those wishing to purchase is $74 for new customers, $39 for upgrade from a previous version of Audirvana Plus, and gratis for those who have purchased Audirvana Plus 2 since December 25, 2016.Īudirvana will not be alone in offering MQA Core decoding. Optimal sound quality is achieved with an MQA-enabled DAC, which will continue to unfold the file "with precise file and platform-specific DAC compensation and management." Clarification on what all this means follows.Īudirvana offers a free 15-day trial version of Audirvana Plus 3. Thus, even without an MQA-enabled DAC, listeners can expect to hear enhanced sound. The MQA Core decoder's initial file "unfold" is claimed to recover all direct music-related information and make it available for either analog or digital output at 88.2 or 96kHz. is anywhere near as extensive as what Roon currently offers is another matter. Whether Audirvana's latest library management system etc. In addition to optimizing sound quality and authenticating file provenance, Audirvana Plus 3 claims to include "a complete library management system with full metadata and cover art display" for music that is either stored locally or streamed, and integration with premium streaming services such as Tidal and Qobuz. 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While there has been significant work performed in generating fibers and materials from recombinant spider silk polypeptides using traditional spinning and molding processes (see U.S. Specifically, silk proteins form complex beta-sheet structures that are extremely stable and only denature at very high temperatures, far above the melting temperature of the protein. Silk proteins such as silk fibroin and spidroins have a complex secondary and tertiary structures which make them an ideal candidate for the creation of protein-based materials. Accordingly, innovations related to novel fibers or materials comprising proteins typically entail precise characterization of any plasticizers used and rheological analysis of the protein composition used to form the materials. For example, plasticizers may be used to lower the glass transition and melting temperature of a protein, thus allowing the protein to be manipulated and reformed into new tertiary structures while preventing degradation of the protein.Ī substantial limitation in using regenerated and recombinant protein sources to create materials is variability in purity of the protein and the presence of impurities such as lipids and sugars which may act as plasticizers, thus producing materials with variable properties. Plasticizers interact with the tertiary structures of proteins and can alter the physio-chemical properties of the proteins. Fiber made from regenerated protein dates back to the 1890s and has been made using various traditional wet-spinning techniques.īecause proteins typically form brittle structures, plasticizers such as polyols, lipids and sugars have been combined with regenerated protein sources to generate fibers and materials that are less susceptible to breaking and deformation. To this end, considerable effort has been made to develop methods of making materials and fibers from regenerated protein sources derived from plants (e.g., zein, soy, wheat gluten) and animals (e.g. Protein-based materials are of increasing interest as an alternative to petroleumbased products. The present invention relates to scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber. 16, 2021, is named BTT-014C1_CRF_sequencelisting.txt and is 61,803 bytes in size. 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The Neighborhood lakes feature parks, picnic tables, parking, and restrooms. However, your kids aged 16 and under get to fish for free. The Neighborhood lakes are in the following towns and Cities: Amarillo, Wichita Falls, Denton, Hurst, Fort Worth, Duncanville, Mesquite City, San Angelo, Abilene, College Station, Waco, San Angelo, Katy, Spring, Pasadena, Missouri City and two lakes in San Antonio.Īs an adult, you need to have a license to fish for trout in any of the Neighborhood lakes. ![]() The TPWD stocks these lakes with Rainbow trout between November and March at intervals of 2 weeks. They offer the convenience of location as they are distributed throughout the Lone Star State. The Neighborhood LakesĪre you looking to fish in the winter or during cooler months? Are you interested in catching Rainbow trout? The 18 Texas Neighborhood lakes are the best place to start. You may also need to use a watercraft without a motor such as a canoe or a raft. 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The Guadalupe trail race has been a major source of freshwater trout For years. You will also be able to utilize the colder periods of the day, such as dusk and dawn to catch trout. You will be able to set up camp and take a couple of days of fishing. Garner State Park has become a popular spot for families. It features numerous public access points for fishing and has beautiful scenery. You will find this rainbow trout habitat eight miles to the north of Concan City. Garner State Park is accommodative of all expert levels, including beginners like your kids. What’s more, the TPWD stocks it on an annual basis. The Frio River delivers freshwater into Garner State Park. ![]() You only need to hire fishing gear if you forgot or failed to bring your own. The first thing I like about fishing in Texas state parks is that fishing is free. 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