![]() ![]() Accordingly, there is a need for processes of manufacturing materials from recombinant spider silk polypeptides which use the specific physio-chemical properties of recombinant spider silk polypeptide compositions to create fibers comprising stable and reproducible tertiary structures. Since impurities and ambient water bound to the protein may plasticize or otherwise interact with the recombinant spider silk polypeptides, failure to understand the impact of these components can produce materials with varying tertiary structures and mechanical properties. Specifically, none of the work has precisely characterized the recombinant spider silk polypeptide that is used as an input to these processes and the molecular structures formed by the recombinant spider silk polypeptide in the material that is output from the processes. 7,057,023), much of this work has been proof-of-principle work that is not reproducible or scalable for mass commercialization. While there has been significant work performed in generating fibers and materials from recombinant spider silk polypeptides using traditional spinning and molding processes (see U.S. Specifically, silk proteins form complex beta-sheet structures that are extremely stable and only denature at very high temperatures, far above the melting temperature of the protein. Silk proteins such as silk fibroin and spidroins have a complex secondary and tertiary structures which make them an ideal candidate for the creation of protein-based materials. Accordingly, innovations related to novel fibers or materials comprising proteins typically entail precise characterization of any plasticizers used and rheological analysis of the protein composition used to form the materials. For example, plasticizers may be used to lower the glass transition and melting temperature of a protein, thus allowing the protein to be manipulated and reformed into new tertiary structures while preventing degradation of the protein.Ī substantial limitation in using regenerated and recombinant protein sources to create materials is variability in purity of the protein and the presence of impurities such as lipids and sugars which may act as plasticizers, thus producing materials with variable properties. Plasticizers interact with the tertiary structures of proteins and can alter the physio-chemical properties of the proteins. Fiber made from regenerated protein dates back to the 1890s and has been made using various traditional wet-spinning techniques.īecause proteins typically form brittle structures, plasticizers such as polyols, lipids and sugars have been combined with regenerated protein sources to generate fibers and materials that are less susceptible to breaking and deformation. To this end, considerable effort has been made to develop methods of making materials and fibers from regenerated protein sources derived from plants (e.g., zein, soy, wheat gluten) and animals (e.g. Protein-based materials are of increasing interest as an alternative to petroleumbased products. The present invention relates to scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber. 16, 2021, is named BTT-014C1_CRF_sequencelisting.txt and is 61,803 bytes in size. The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. 25, 2017, the contents of which are incorporated by reference in its entirety. 25, 2018, which claims the benefit of U.S. ![]() This application is a continuation of U.S. Patent Description CROSS-REFERENCE TO RELATED APPLICATIONS
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